Browsing Requests for Comments (RFCs) by Author "Adlung, Lorenz"

Now showing items 1-4 of 4

    • HiCT: High Throughput Protocols For CPE Cloning And Transformation 

      Beer, Ralf; Christiansen, Tania; Herbst, Konrad; Ignatiadis, Nikolaos; Kats, Ilia; e.a. (2013-10-04)
      The purpose of this RFC is to provide instructions for a rapid and cost efficient cloning and transformation method which allows for the manufacturing of multi-fragment plasmid constructs in a parallelized manner: High ...

    • miMeasure – a standard for miRNA binding site characterization in mammalian cells 

      Adlung, Lorenz; Sabah, Jude Al; Bayer, Philipp; Berrens, Rebecca; Cristiano, Elena; e.a. (2010-12-05)
      This RFC proposes a standard for the quantitative characterization of miRNA binding sites (miRNA-BS) in mammalian cells. The miMeasure standard introduces a ready-to-use standard measurement plasmid (pSMB_miMeasure, ...

    • miTuner - a kit for microRNA based gene expression tuning in mammalian cells 

      Adlung, Lorenz; Sabah, Jude Al; Bayer, Philipp; Berrens, Rebecca; Cristiano, Elena; e.a. (2010-12-05)
      The purpose of this RFC is to introduce a modular expression tuning kit for use in mammalian cells. The kit enables the regulation of the gene expression of any gene of interest (GOI) based on synthetic microRNAs, endogenous ...

    • Standard for Synthesis of Customized Peptides by Non-Ribosomal Peptide Synthetases 

      Beer, Ralf; Christiansen, Tania; Herbst, Konrad; Ignatiadis, Nikos; Kats, Ilia; e.a. (2013-10-04)
      The purpose of this RFC is to introduce a standardized framework for the engineering of customizable non-ribosomal peptide synthetases (NRPS) and their application for in vivo and in vitro synthesis of short non-ribosomal ...