Browsing Requests for Comments (RFCs) by Issue Date

Now showing items 1-20 of 74

    • Draft Standard for Biobrick Biological Parts 

      Knight, Tom (OpenWetWare, 2007-05-03)
      This standard defines the required sequence properties for a Biobrick(tm) standard biological part. It does not define any functional characteristics of the parts, nor does it motivate any aspect of these standards. ...

    • Instructions to BBF RFC Authors 

      Tarjan, Daniel; Sleight, Sean; Shetty, Reshma; Ridgway, Doug; Rettberg, Randy; e.a. (OpenWetWare, 2008-11-10)
      This BioBricks Foundation Request for Comments (BBF RFC) provides in- formation about the preparation and submission of BBF RFCs to The Bio- Bricks Foundation (BBF).

    • Draft Standard for Biobrick BB-2 Biological Parts 

      Knight, Tom (OpenWetWare, 2008-11-19)
      This standard defines the required sequence properties for a Biobrick(R) BB-2 standard biological part. It does not define any functional characteristics of the parts, nor does it motivate any aspect of these standards.

    • Proposed Conceptual Guidelines for the Design of a BioBrick Graphical Language & an Example 

      Fernandez, Eric (2009-03-12)
      As we update the current BioBrick symbols we have an opportunity to look at the end users of a BioBrick Graphical language and develop design guidelines so that the new symbols best suit their needs. This document explores ...

    • Conversion of Freiburg (Fusion) Biobricks to the Silver (BioFusion) format 

      Grunberg, Raik (2009-03-30)
      This Request for Comments (RFC) describes a strategy for converting Biobricks from the Freiburg (aka Fusion) format to the Silver lab (aka BioFusion) format.

    • Constraint Relaxation of RFC 10 for Assembling Standard Biological Parts 

      Che, Austin (2009-03-30)
      This Request for Comments (RFC) modifies the assembly standard for biological parts proposed in BBF RFC 10 by replacing PstI with SbfI and dropping other sequence constraints. This change relaxes the constraints on the ...

    • Naming of standards of physical composition of BioBrick parts 

      Rettberg, Randy; Shetty, Reshma (2009-04-17)
      This BioBricks Foundation Request for Comments (BBF RFC) proposes a naming convention for standards of physical composition of BioBrick parts in which assembly standards are named according to the BBF RFC that specifies ...

    • Fusion Protein (Freiburg) Biobrick assembly standard 

      Grünberg, Raik; Arndt, Katja; Müller, Kristian (2009-04-18)
      This Request for Comments (RFC) describes an extension to the original BioBrick assembly standard (BBF RFC 10). The Fusion Assembly strategy described here is fully backwards compatible with RFC 10 and supports the ...

    • BBF RFC 30: Draft of an RDF-based framework for the exchange and integration of Synthetic Biology data 

      Grunberg, Raik (2009-04-24)
      This Request for Comments (RFC) suggests a framework for the description, exchange and interlinking of Synthetic Biology data. The framework would create an open process for the evolution and “rolling” standardization ...

    • Provisional BioBrick Language (PoBoL) 

      Galdzicki, Michal; Chandran, Deepak; Nielsen, Alec; Morrison, Jason; Cowell, Mackenzie; e.a. (2009-05-15)
      This BioBricks Foundation Request for Comments (BBF RFC) describes a semantic markup language for publishing and sharing information about BioBricks on the World Wide Web. This BBF RFC includes the recommendation for the ...

    • BBF RFC 26: In-Fusion BioBrick Assembly 

      Sleight, Sean (2009-07-09)
      This BioBricks Foundation Request for Comments (BBF RFC) describes a method to assemble two BioBricks using the Clontech In-Fusion PCR Cloning Kit while maintaining BioBrick standard formats.

    • BBF RFC 36: Fusion protein BioBrick assembly standard with optional linker extension 

      Jerala, Roman (2009-09-01)
      This RFC 36 describes an extension of the original BioBrick assembly standard (BBF RFC 10) and Freiburg assembly standard (BBF RFC 25). The Fusion Assembly strategy described here is fully compatible with RFC 25 (Freiburg) ...

    • BBF RFC 37: Fusion protein BioBrick assembly standard with optional linker extension 

      Bencina, Mojca; Jerala, Roman (2009-09-02)
      This RFC 37 describes an extension of the original BioBrick assembly standard (BBF RFC 10) and Freiburg assembly standard (BBF RFC 25). The Fusion Assembly strategy described here is fully compatible with RFC 25 (Freiburg) ...

    • BBF RFC 28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI 

      Peisajovich, Sergio G.; Horwitz, Andrew; Hoeller, Oliver; Rhau, Benjamin; Lim, Wendell (2009-09-16)
      This BioBricks Foundation Request for Comments (BBF RFC) describes an alternative assembly standard based on the Type IIS restriction enzyme AarI.

    • BBF RFC 21: BglBricks Assembly Standard 

      Anderson, J Christopher; Dueber, John E; Leguia, Mariana; Wu, Gabriel C; Goler, Jonathan A; e.a. (2009-09-18)
      The BglBricks standard has been developed as an alternative to the original XbaI/SpeI standard primarily to provide a solution to the issue of generating translational-fusion parts. When joined together in a standard ...

    • BBF RFC 38: RFC 38: Building Blocks - Standard Large DNA/Genome Construction 

      DiCarlo, James; Boeke, Jef (2009-10-07)
      The physical assembly of standard parts is currently a non-standard process, which can either come from direct genome PCR with restriction enzyme sites incorporated into the PCR primers, or overlap assembly PCR. ...

    • Units for Promoter Measurement in Mammalian Cells 

      Velten, Lars; Iwamoto, Nao; Hiller, Corinna; Uckelmann, Hannah; Zhu, Chenchen; e.a. (2009-10-21)
      The purpose of this RFC is to provide units for the characterization of promoter strength for use in mammalian cells. RMPU is mRNA based and directly proportional to PoPS, whereas REU is protein based and not proportional ...

    • RA-PCR, a method for the generation of randomized promoter libraries 

      Velten, Lars; Haas, Simon; Rademacher, Anne; Meyer, Hannah; Eils, Roland (2009-10-21)
      The purpose of this RFC is to provide instructions for the synthesis of promoters in mammalian cells that are active at a desired cellular condition (where a cellular condition is specified by the activity of a set of ...

    • Cloning Standard for Mammalian BioBrick Parts and Devices 

      Bartoschek, Michael; Mugahid, Douaa; Rademacher, Anne; Meyer, Hannah; Velten, Lars; e.a. (2009-10-21)
      To introduce a common cloning standard for BioBrick parts that find application in mammalian cells.

    • How to Build Kinetic Models of BioBricks 

      Weeding, Emma; Houle, Jason; Swiniarski, Ben; Smadbeck, Patrick; Lindblad, Kristen; e.a. (2009-10-21)
      This BioBricks Foundation Request for Comments (BBF RFC) provides instructions on how to automatically generate a model of any BioBrick sequence. These models can be used in computer simulations of the dynamic behavior of ...