BBF RFC 36: Fusion protein BioBrick assembly standard with optional linker extension

Abstract

This RFC 36 describes an extension of the original BioBrick assembly standard (BBF RFC 10) and Freiburg assembly standard (BBF RFC 25). The Fusion Assembly strategy described here is fully compatible with RFC 25 (Freiburg) and RFC 10 standard and supports in-frame fusion and controlled potentially infinite extension of linker between protein fusion domains.