Browsing MIT Open Access Articles by Author "Aach, John"

Now showing items 1-4 of 4

    • Barcoded oligonucleotides ligated on RNA amplified for multiplexed and parallel in situ analyses 

      Liu, Songlei; Punthambaker, Sukanya; Iyer, Eswar PR; Ferrante, Thomas; Goodwin, Daniel; e.a. (Oxford University Press (OUP), 2021)
      <jats:title>Abstract</jats:title> <jats:p>We present barcoded oligonucleotides ligated on RNA amplified for multiplexed and parallel insitu analyses (BOLORAMIS), a reverse transcription-free method for ...

    • Characterizing the portability of phage-encoded homologous recombination proteins 

      Filsinger, Gabriel T; Wannier, Timothy M; Pedersen, Felix B; Lutz, Isaac D; Zhang, Julie; e.a. (Springer Science and Business Media LLC, 2021)
      © 2021, The Author(s), under exclusive licence to Springer Nature America, Inc. Efficient genome editing methods are essential for biotechnology and fundamental research. Homologous recombination (HR) is the most versatile ...

    • Engineering and optimising deaminase fusions for genome editing 

      Yang, Luhan; Briggs, Adrian W.; Chew, Wei Leong; Mali, Prashant; Guell, Marc; e.a. (Nature Publishing Group, 2016-11)
      Precise editing is essential for biomedical research and gene therapy. Yet, homology-directed genome modification is limited by the requirements for genomic lesions, homology donors and the endogenous DNA repair machinery. ...

    • Highly efficient Cas9-mediated transcriptional programming 

      Chavez, Alejandro; Scheiman, Jonathan; Tuttle, Marcelle; Lin, Shuailiang; Kiani, Samira; e.a. (Nature Publishing Group, 2015-03)
      The RNA-guided nuclease Cas9 can be reengineered as a programmable transcription factor. However, modest levels of gene activation have limited potential applications. We describe an improved transcriptional regulator ...