Highly efficient Cas9-mediated transcriptional programming

Author(s)
Chavez, Alejandro; Scheiman, Jonathan; Tuttle, Marcelle; Lin, Shuailiang; Kiani, Samira; ... Show more
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Abstract
The RNA-guided nuclease Cas9 can be reengineered as a programmable transcription factor. However, modest levels of gene activation have limited potential applications. We describe an improved transcriptional regulator obtained through the rational design of a tripartite activator, VP64-p65-Rta (VPR), fused to nuclease-null Cas9. We demonstrate its utility in activating endogenous coding and noncoding genes, targeting several genes simultaneously and stimulating neuronal differentiation of human induced pluripotent stem cells (iPSCs).
Date issued
2015-03
URI
http://hdl.handle.net/1721.1/99529
Department
Massachusetts Institute of Technology. Institute for Medical Engineering & Science; Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science; Massachusetts Institute of Technology. Synthetic Biology Center
Journal
Nature Methods
Publisher
Nature Publishing Group
Citation
Chavez, Alejandro, Jonathan Scheiman, Suhani Vora, Benjamin W Pruitt, Marcelle Tuttle, Eswar P R Iyer, Shuailiang Lin, et al. “Highly Efficient Cas9-Mediated Transcriptional Programming.” Nat Meth 12, no. 4 (March 2, 2015): 326–328.
Version: Author's final manuscript
ISSN
1548-7091
1548-7105
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