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Highly efficient Cas9-mediated transcriptional programming

dc.contributor.authorChavez, Alejandro
dc.contributor.authorScheiman, Jonathan
dc.contributor.authorTuttle, Marcelle
dc.contributor.authorLin, Shuailiang
dc.contributor.authorKiani, Samira
dc.contributor.authorTer-Ovanesyan, Dmitry
dc.contributor.authorDavidsohn, Noah
dc.contributor.authorPerrimon, Norbert
dc.contributor.authorWeiss, Ron
dc.contributor.authorAach, John
dc.contributor.authorVora, Suhani Deepak
dc.contributor.authorPruitt, Benjamin W.
dc.contributor.authorIyer, Eswar P. R.
dc.contributor.authorGuzman, Christopher D.
dc.contributor.authorWiegand, Daniel J.
dc.contributor.authorBraff, Jonathan L.
dc.contributor.authorHousden, Benjamin E.
dc.contributor.authorCollins, James J.
dc.contributor.authorChurch, George M.
dc.date.accessioned2015-10-30T17:46:23Z
dc.date.available2015-10-30T17:46:23Z
dc.date.issued2015-03
dc.date.submitted2014-12
dc.identifier.issn1548-7091
dc.identifier.issn1548-7105
dc.identifier.urihttp://hdl.handle.net/1721.1/99529
dc.description.abstractThe RNA-guided nuclease Cas9 can be reengineered as a programmable transcription factor. However, modest levels of gene activation have limited potential applications. We describe an improved transcriptional regulator obtained through the rational design of a tripartite activator, VP64-p65-Rta (VPR), fused to nuclease-null Cas9. We demonstrate its utility in activating endogenous coding and noncoding genes, targeting several genes simultaneously and stimulating neuronal differentiation of human induced pluripotent stem cells (iPSCs).en_US
dc.description.sponsorshipNational Human Genome Research Institute (U.S.) (Grant P50 HG005550)en_US
dc.description.sponsorshipUnited States. Dept. of Energy (Grant DE-FG02-02ER63445)en_US
dc.description.sponsorshipWyss Institute for Biologically Inspired Engineeringen_US
dc.description.sponsorshipNational Science Foundation (U.S.). Graduate Research Fellowshipen_US
dc.description.sponsorshipMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.description.sponsorshipHarvard Medical School. Department of Geneticsen_US
dc.language.isoen_US
dc.publisherNature Publishing Groupen_US
dc.relation.isversionofhttp://dx.doi.org/10.1038/nmeth.3312en_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourcePMCen_US
dc.titleHighly efficient Cas9-mediated transcriptional programmingen_US
dc.typeArticleen_US
dc.identifier.citationChavez, Alejandro, Jonathan Scheiman, Suhani Vora, Benjamin W Pruitt, Marcelle Tuttle, Eswar P R Iyer, Shuailiang Lin, et al. “Highly Efficient Cas9-Mediated Transcriptional Programming.” Nat Meth 12, no. 4 (March 2, 2015): 326–328.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Institute for Medical Engineering & Scienceen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Electrical Engineering and Computer Scienceen_US
dc.contributor.departmentMassachusetts Institute of Technology. Synthetic Biology Centeren_US
dc.contributor.mitauthorVora, Suhani Deepaken_US
dc.contributor.mitauthorKiani, Samiraen_US
dc.contributor.mitauthorWeiss, Ronen_US
dc.contributor.mitauthorCollins, James J.en_US
dc.relation.journalNature Methodsen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsChavez, Alejandro; Scheiman, Jonathan; Vora, Suhani; Pruitt, Benjamin W; Tuttle, Marcelle; P R Iyer, Eswar; Lin, Shuailiang; Kiani, Samira; Guzman, Christopher D; Wiegand, Daniel J; Ter-Ovanesyan, Dmitry; Braff, Jonathan L; Davidsohn, Noah; Housden, Benjamin E; Perrimon, Norbert; Weiss, Ron; Aach, John; Collins, James J; Church, George Men_US
dc.identifier.orcidhttps://orcid.org/0000-0003-0396-2443
dc.identifier.orcidhttps://orcid.org/0000-0003-1736-0937
dc.identifier.orcidhttps://orcid.org/0000-0002-5560-8246
mit.licensePUBLISHER_POLICYen_US
mit.metadata.statusComplete


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