dc.contributor.author | Shi, Zhenyu | |
dc.contributor.author | Li, Teng | |
dc.contributor.author | Chen, Guoqiang | |
dc.date.accessioned | 2010-10-31T21:01:48Z | |
dc.date.available | 2010-10-31T21:01:48Z | |
dc.date.issued | 2010-10-31 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/59802 | |
dc.description.abstract | With the established BioBrick Assembly standards, ligation of different parts has to be accomplished step by step. It can be time-consuming when dealing with multiple fragment ligation. BBF RFC 61 is developed aimed at completing the ligation of multiple fragments quickly and efficiently based on Type II restriction enzyme DraIII. | en_US |
dc.language.iso | en | en_US |
dc.relation.ispartofseries | BBF RFC;61 | |
dc.rights | Attribution-No Derivative Works 3.0 United States | en |
dc.rights.uri | http://creativecommons.org/licenses/by-nd/3.0/us/ | en |
dc.subject | assembly | en_US |
dc.subject | method | en_US |
dc.title | Fast multiple gene fragment ligation method based on Type IIs restriction enzyme DraIII | en_US |
dc.type | Technical Report | en_US |