Fast multiple gene fragment ligation method based on homologous recombination

Author(s)
Wang, Ruiyan; Shi, Zhenyu; Li, Teng; Chen, Guoqiang
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Attribution-No Derivative Works 3.0 United States http://creativecommons.org/licenses/by-nd/3.0/us/
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Abstract
With the established BioBrick Assembly standards, ligation of different parts has to be accomplished step by step. It can be time-consuming when dealing with multiple fragment ligation. BBF RFC 62 is developed aimed at completing the ligation of multiple fragments quickly and efficiently based on homologous recombination.
Date issued
2010-10-31
URI
http://hdl.handle.net/1721.1/59803
Series/Report no.
BBF RFC;62
Keywords
assembly, method
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