Promoter and Coding Sequence Considerations for Caenorhabditis elegans and Other Eukaryotes

Abstract

Our primary aim is to provide a standard format for protein-coding BioBricks intended for eukaryotes, especially in the nematode worm Caenorhabditis elegans, so that future teams will be able to correctly take advantage of the Kozak consensus sequence and its power to manipulate gene expression levels. There are currently, in fact, at least three protein-coding BioBricks with Kozak sequences that correctly conform to this convention, designed for yeast, (BBa_K165057, BBa_K165058, and BBa_K165059) but their format has not been documented or standardized. Since this is so important to full exploitation of current and future eukaryotic chassis, we feel it must be codified.