PCR - Ligation Assembly Standard for BioBrick Parts

Author(s)
He, Tony PeiYuan; Campbell, Alexander; Zhou, Stefanie Yifan
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Attribution-NoDerivs 3.0 United States http://creativecommons.org/licenses/by-nd/3.0/us/
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Abstract
This Request for Comments (RFC) describes a novel method for the assembly of standard BioBrick parts. This assembly method for BioBrick parts is an improvement upon the conventional methods of BioBrick part assembly. This assembly method will involve the use of standard BioBrick restriction enzyme systems, PCR purification kit, ligase system, and a PCR polymerase system.
Date issued
2011-12-15
URI
http://hdl.handle.net/1721.1/67700
Series/Report no.
BBF RFC;83
Keywords
DNA assembly
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