BBF RFC 104: BrickClip – rapid assembly of multiple RFC10 BioBricks

Abstract

This BioBrick Foundation Request for Comments (BBF RFC 104) describes a new approach to multiple part DNA assembly – BrickClip, which does not require use of any restriction enzymes, nor cloning of the parts to specific donor and acceptor vectors. BrickClip allows assembly of up to six parts from existing parts collections, including the Registry of Standard Biological Parts, in a single reaction, in any desired order. The resulting product is exactly the same as would be obtained from normal RFC10 BioBrick assembly. In contrast to other commonly used methods such as Gibson assembly, BrickClip does not require ordering new oligonucleotides for each assembly; while assembly oligonucleotides are required, these are part-specific and may be re-used in any assembly involving a given part. BrickClip is a special case of a more general assembly method – PaperClip.