Browsing Molecular Engineering of Biological and Chemical Systems (MEBCS) by Title
Now showing items 1-20 of 106
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Activation Ratios For Reconstruction Of Signal Transduction Networks
(2003-01)We have developed a novel framework that can be applied for the analysis of signal transduction networks, both to facilitate reconstruction of the network structure and quantitatively characterize the interaction between ... -
Active Hypothermic Growth: A Novel Means For Increasing Total Interferon-γ Production by Chinese Hamster Ovary Cells
(2006-01)When grown under hypothermic conditions, Chinese Hamster Ovary (CHO) cells become growth arrested in the G₀/G₁ phase of the cell cycle and also often exhibit increased recombinant protein production. In this study, we ... -
Apatite-Polymer Composite Particles for Controlled Delivery of BMP-2
(2004-01)We have developed a versatile delivery platform comprising a novel composite of two biomaterials with proven track records: apatite and poly(lactic-co-glycolic acid) (PLGA). These composites have been tested in the delivery ... -
Apatite-Polymer Composite Particles for Controlled Delivery of BMP-2: In Vitro Release and Cellular Response
(2005-01)Bone morphogenetic protein-2 (BMP-2) has the ability to induce osteoblast differentiation of undifferentiated cells, resulting in the healing of skeletal defects when delivered with a suitable carrier. We have applied a ... -
Apatite-Polymer Composites for the Controlled Dual Delivery of BMP-2 and BMP-6 for Bone Tissue Engineering
(2005-01)The release of growth factors from tissue engineering scaffolds provides signals that influence the migration, differentiation, and proliferation of cells. The incorporation of a drug delivery platform that is capable of ... -
Association Behavior of Biotinylated and Non-Biotinylated PolyEthylene Oxide-b-Poly(2-(Diethylamino)Ethyl Methacrylate)
(2005-01)Biotinylated and non-biotinylated copolymers of ethylene oxide (EO) and 2-(diethylamino)ethyl methacrylate (DEAEMA) were synthesized by the atom transfer radical polymerization technique (ATRP). The chemical compositions ... -
Association Behavior of Poly (methyl methacrylate-b-methacrylic acid-b-methyl methacrylate) in Aqueous Medium
(2004-01)ABA type tri-block amphiphilic polyelectrolyte consisting of poly(methyl methacrylate-block-methacrylic acid-block-methyl methacrylate) (P(MMA-b-MAA-b-MMA)) was synthesized by atom transfer radical polymerization technique ... -
Association Behavior of Poly(methacrylic acid)-block-Poly(methyl methacrylate) in Aqueous Medium: Potentiometric and Laser Light Scattering Studies
(2003-01)Atom transfer radical polymerisation (ATRP) technique was used to synthesize poly(methacrylic acid-block-methyl methacrylate) (P(MAA₁₀₂-b-MMA₁₀)) copolymer in order to study the aggregation behavior in aqueous solution ... -
Bioinformatics and Handwriting/Speech Reconition: Uncoventional Applications of Similarity Search Tools
(2006-01)This work introduces two unconventional applications for sequence alignment algorithms outside the domain of bioinformatics: handwriting recognition and speech recognition. In each application we treated data samples, such ... -
Biological Routes to Gold Nanoplates
(2005-01)Much effort has been devoted to the synthesis of gold nanoparticles with different shapes, including the zero-dimensional nanospheres, one dimensional nanorods, and two-dimensional nanoplates. Compared to zero or one ... -
Bubble Behavior in a Taylor Vortex
(2005-01)We present an experimental study on the behavior of bubbles captured in a Taylor vortex. The gap between a rotating inner cylinder and a stationary outer cylinder is filled with a Newtonian mineral oil. Beyond a critical ... -
Cell-line Engineering for Low-temperature Growth
(2004-01)In the chemical industry, the rates of reactions are usually enhanced by the use of high-temperature and high-pressure conditions. This chemical engineering approach is rarely applied in the biotechnology field. Firstly, ... -
Cell-line Engineering of Chinese Hamster Ovary Cells for Low-temperature Culture
(2005-01)Developments in mammalian cell culture and recombinant technology has allowed for the production of recombinant proteins for use as human therapeutics. Mammalian cell culture is typically operated at the physiological ... -
Changes in the Overall Extent of Protein Glycosylation by Chinese Hamster Ovary Cells Over the Course of Batch Culture
(2003-01)Glycosylation of recombinant proteins can change during the culture of animal cells. Since lipid-linked oligosaccharides (LLOs) are the carbohydrate donors in N-linked glycosylation, their availability is postulated to ... -
CIRP Expression on Growth and Productivity of CHO Cells
(2006-01)Mammalian cell culture is typically operated at the physiological temperature of 37°C. Low temperature cell culture at 30-33°C, in particular for CHO cells, increased the specific productivity of many recombinant proteins ... -
Computational Method for Drug Target Search and Application in Drug Discovery
(2003-01)Ligand-protein inverse docking has recently been introduced as a computer method for identification of potential protein targets of a drug. A protein structure database is searched to find proteins to which a drug can bind ... -
A Computational Study to Understand the Surface Reactivity of Gold Nanoparticles with Amines and DNA
(2006-01)We conducted a computational adsorption study of methylamine on various surface-models of gold nanoparticle which is facetted by multiple {111} and {100} planes. In addition to these flat surfaces, our models include the ... -
Computer-Aided Drug Target Search
(2003-01)Identification of the unknown targets of drugs, investigative drugs and herbal ingredients is an important task in drug discovery. It can potentially help in several aspects including: (1) determination of unknown therapeutic ... -
Core-Shell Assisted Bimetallic Assembly of Pt and Ru Nanoparticles by DNA Hybridization
(2005-01)We have discovered that the current protocols to assemble Au nanoparticles based on DNA hybridization do not work well with the small metal nanoparticles (e.g. 5 nm Au, 3.6 nm Pt and 3.2 nm Ru particles). Further investigations ... -
Correlating Gene Transfection Efficiency and the Physical Properties of Various Cationic Poly(methacrylate) Systems
(2006-01)Transfection efficiencies of several polymeric gene carriers were compared and correlated quantitatively to the amounts of cellular accumulation of plasmid DNA and to the expression of mRNA by quantitative real time PCR. ...
