Browsing Requests for Comments (RFCs) by Subject "DNA assembly"

Now showing items 1-7 of 7

    • "BioSandwich", a homology-based assembly method using a library of standard parts 

      Abbas, Yassen; Crossman, Allan; Fletcher, Eugene; French, Chris; Gibson, Clare; e.a. (2011-10-17)
      This Request for Comments (RFC) describes a strategy for using homology-based assembly methods to assemble parts from a library in any order.

    • GenBrick – A Rapid Multi-Part Assembly Method for BioBricks 

      Gasiūnaitė, Lina; Lewicka, Aleksandra; Pashkuleva, Hristiana; Villanueva, Hugo; Thornton, Harry; e.a. (2013-10-04)
      This BioBricks Foundation Request for Comments (BBF RFC) describes a novel approach, GenBrick, for the rapid assembly of multiple BioBrick RFC10-compatible parts in a single reaction, with completely flexible part order, ...

    • The GoldenBricks assembly: A standardized one-shot cloning technique for complete cassette assembly 

      Pauthenier, Cyrille; El-Sayyed, Hafez; Rostain, William; Cerisy, Tristan; Lopez, Carolina Gallo; e.a. (2012-11-05)
      BBF RFC 92 proposes a new standard assembly method for the Parts Registry. The method makes one-shot cloning of a complete eukaryotic or prokaryotic cassette possible in one day while keeping compatibility with the BBF RFC ...

    • HiCT: High Throughput Protocols For CPE Cloning And Transformation 

      Beer, Ralf; Christiansen, Tania; Herbst, Konrad; Ignatiadis, Nikolaos; Kats, Ilia; e.a. (2013-10-04)
      The purpose of this RFC is to provide instructions for a rapid and cost efficient cloning and transformation method which allows for the manufacturing of multi-fragment plasmid constructs in a parallelized manner: High ...

    • PCR - Ligation Assembly Standard for BioBrick Parts 

      He, Tony PeiYuan; Campbell, Alexander; Zhou, Stefanie Yifan (2011-12-15)
      This Request for Comments (RFC) describes a novel method for the assembly of standard BioBrick parts. This assembly method for BioBrick parts is an improvement upon the conventional methods of BioBrick part assembly. This ...

    • Reusable rapid assembly of genetic parts for Neurospora crassa 

      Odsen, Raymond; Baretta, Kayla; Zakrewski, Helena; Simpson, Michael; Guo, Yuan; e.a. (2011-10-17)
      This BBF RFC provides a method in which standardized parts can be easily created for Neurospora crassa.

    • Yeast Golden Gate: Standardized Assembly of S. cerevisiae Transcriptional Units 

      Boeke, Jef; Mitchell, Leslie; Cai, Yizhi; Stracquadanio, Giovanni; Chuang, James; e.a. (2012-10-11)
      BBF RFC 88 describes a new standard for the assembly of basic Saccharomyces cerevisiae transcriptional units (TUs) consisting of a promoter/5’untranslated region (UTR), open reading frame (ORF), and 3’UTR/terminator. Note ...