Browsing Requests for Comments (RFCs) by Subject "assembly"

Now showing items 1-8 of 8

    • BBF RFC 104: BrickClip – rapid assembly of multiple RFC10 BioBricks 

      Trubitsyna, Maryia; Chan, Karen; Cai, Yizhi; Elfick, Alistair; French, Chris (2015-03-17)
      This BioBrick Foundation Request for Comments (BBF RFC 104) describes a new approach to multiple part DNA assembly – BrickClip, which does not require use of any restriction enzymes, nor cloning of the parts to specific ...

    • BBF RFC 106: A Standard Type IIS Syntax for Plants 

      Rutten, Virginia; Munabi, Angelina; Riche, Fergus; Lewy, Guy; Wilson, Hugh; e.a. (2015-03-17)
      Here we define a standard syntax for assembling standard parts for expression in plant cells, extensible to all other eukaryotes. Variations of the Type IIS mediated cloning method known as Golden Gate Cloning, most notably ...

    • BBF RFC 28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI 

      Peisajovich, Sergio G.; Horwitz, Andrew; Hoeller, Oliver; Rhau, Benjamin; Lim, Wendell (2009-09-16)
      This BioBricks Foundation Request for Comments (BBF RFC) describes an alternative assembly standard based on the Type IIS restriction enzyme AarI.

    • Bioscaffold-Linker 

      Mina, Petros; Savery, Nigel (2009-10-21)
      Protein fusions are currently not supported by most assembly standards. This assembly standard aims to provide the basis of a solution to a biobrick-friendly protein fusion mechanism that supports the current favorite ...

    • Cloning Standard for Mammalian BioBrick Parts and Devices 

      Bartoschek, Michael; Mugahid, Douaa; Rademacher, Anne; Meyer, Hannah; Velten, Lars; e.a. (2009-10-21)
      To introduce a common cloning standard for BioBrick parts that find application in mammalian cells.

    • Fast multiple gene fragment ligation method based on homologous recombination 

      Wang, Ruiyan; Shi, Zhenyu; Li, Teng; Chen, Guoqiang (2010-10-31)
      With the established BioBrick Assembly standards, ligation of different parts has to be accomplished step by step. It can be time-consuming when dealing with multiple fragment ligation. BBF RFC 62 is developed aimed at ...

    • Fast multiple gene fragment ligation method based on Type IIs restriction enzyme DraIII 

      Shi, Zhenyu; Li, Teng; Chen, Guoqiang (2010-10-31)
      With the established BioBrick Assembly standards, ligation of different parts has to be accomplished step by step. It can be time-consuming when dealing with multiple fragment ligation. BBF RFC 61 is developed aimed at ...

    • The USER cloning standard 

      Kaas, Christian Schrøder; Hansen, Niels Bjørn; Genee, Hans Jasper; Olsen, Lars Rønn; Matos, Cláudia; e.a. (2009-11-01)
      This BioBricks Foundation Request for Comments (BBF RFC) provides information about the design of uracil-containing primers used for USER cloning and USER fusion.