Novel Normalization Standard using Fluorescence

Author(s)
Bukhari, Syed Habib Tahir; Akkineni, Ashwini Rahul; Ananth, Adithya; Gordeev, Victor; Grieb, Svea; ... Show more
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Attribution-No Derivative Works 3.0 United States http://creativecommons.org/licenses/by-nd/3.0/us/
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Abstract
The Biotec_Dresden Team 2010 developed an approach where two fluorescent proteins are simultaneously expressed. The fact that one reporter, in our case RFP, is constitutively expressed allows to monitor cell growth. Secondly, an inducible promoter drives the expression of the second reporter, YFP in the case of the part tested. The constitutively expressed reporter protein (R1) serves as normalization factor for the inducible reporter (R2) by simple division.
Date issued
2010-12-05
URI
http://hdl.handle.net/1721.1/60087
Series/Report no.
BBF RFC;78
Keywords
measurement standard
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