dc.contributor.author | Bukhari, Syed Habib Tahir | |
dc.contributor.author | Akkineni, Ashwini Rahul | |
dc.contributor.author | Ananth, Adithya | |
dc.contributor.author | Gordeev, Victor | |
dc.contributor.author | Grieb, Svea | |
dc.contributor.author | Mansour, Sarah | |
dc.contributor.author | Roth, Mareike | |
dc.contributor.author | Sampathkumar, Charanya | |
dc.contributor.author | Schirmer, Lucas | |
dc.contributor.author | Tam, Jonathan | |
dc.date.accessioned | 2010-12-05T20:57:12Z | |
dc.date.available | 2010-12-05T20:57:12Z | |
dc.date.issued | 2010-12-05 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/60087 | |
dc.description.abstract | The Biotec_Dresden Team 2010 developed an approach where two fluorescent proteins are simultaneously expressed. The fact that one reporter, in our case RFP, is constitutively expressed allows to monitor cell growth. Secondly, an inducible promoter drives the expression of the second reporter, YFP in the case of the part tested. The constitutively expressed reporter protein (R1) serves as normalization factor for the inducible reporter (R2) by simple division. | en_US |
dc.language.iso | en | en_US |
dc.relation.ispartofseries | BBF RFC;78 | |
dc.rights | Attribution-No Derivative Works 3.0 United States | en |
dc.rights.uri | http://creativecommons.org/licenses/by-nd/3.0/us/ | en |
dc.subject | measurement standard | en_US |
dc.title | Novel Normalization Standard using Fluorescence | en_US |
dc.type | Technical Report | en_US |